Since its opening, the Jerusalem Biblical Zoo has been operated by a company that was specifically established for this purpose. The zoo is therefore being run as a corporation, while at the same time being recognized as an NPO (Non-Profit Organization) both in Israel and abroad. The zoo is the product of the combined efforts and investments of the Municipality of Jerusalem, the Jerusalem Foundation, the Jerusalem Development Authority, and the Israel Ministry of Tourism.

The ownership of the zoo is shared and divided evenly among the Municipality of Jerusalem, the Jerusalem Foundation, and the Jerusalem Development Authority. The zoo's Board of Directors consists of 18 members.

The establishment of the zoo in its present form was made possible thanks to the generosity of Tisch family of New York, under the auspices of the Jerusalem Foundation. The dedication ceremony took place in the presence of the President of the State of Israel at the time, the late Chaim Herzog, and members of the Tisch family, in November 1992.

On February 28, 1993, the zoo opened its doors to the public for a preview period. The official opening ceremony took place on September 9, 1993.

The zoo was designed by Miller-Blum & Associates Landscape Architects, and was constructed by the Moriah - Jerusalem Development Corporation. Since its opening, the zoo has added many new exhibits and features. Thanks mostly to the efforts of the Jerusalem Foundation, we were fortunate to receive the generous donations that were needed for each addition, so that the Jerusalem Biblical Zoo could develop into the institution that it is today.